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AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using <t>ELISA.</t> (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.
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AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.

Journal: Biochemistry and Biophysics Reports

Article Title: Screening of metabolic-related biomarkers linking intervertebral disc degeneration and type 2 diabetes based on comprehensive bioinformatics analysis and machine learning

doi: 10.1016/j.bbrep.2026.102593

Figure Lengend Snippet: AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.

Article Snippet: Cytokine quantification was performed using a commercial double-antibody sandwich ELISA kit (Elabscience, E-EL-H0315, AMBP) according to the manufacturer's instructions.

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing